CASP3F
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 | Caspase 3 activity in lysates of Jurkat cells induced with staursporine Jurkat cells were induced with 1 μg/ml staurosporine for 0, 1, 2, and 3 hours. At the end of the time, the cells were collected and lysed using 500 μl lysis buffer per 107 cells. Caspase 3 activity per 5 μl of either cell lysate or 0.5 μg/ml caspase 3 positive control solution (C) was measured fluorometrically in 96 well multiwell plates. The reaction volume was 200 μl containing 16.6 μM Ac-DEVD-AMC in 1X assay buffer. The assay was performed at room temperature, and readings were taken every 15 minutes, starting at 25 minutes. |
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CASP3F
Sigma
Caspase 3 Assay Kit, Fluorimetric
Description
| Unit Definition | One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C. |
| General description | The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit). |
Properties
| usage | sufficient for 1,000 multiwell tests |
| sufficient for 100 standard tests (2 ml) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
Components
Related Products
| Related product | P8340, Protease Inhibitor Cocktail |
